Modification of tissue factor by peroxynitrite influences its procoagulant activity

Expression of tissue factor procoagulant activity: regulation by cytosolic calcium.

Intact bovine fibroblasts, pericytes, and kidney cells manifested significantly less tissue factor procoagulant activity than their disrupted counterparts. Addition of calcium ionophore A23187 rapidly and reversibly enhanced the cell-surface expression of tissue factor in intact cells up to the level achieved by disruption. Inhibitors of calmodulin blocked the ionophore-dependent enhancement of...

Destruction of factor VIII procoagulant activity in tissue culture media.

Human endothelial cells have been repoded to synthesize protein that has von Willebrand factor activity and reacts with a heterologous antibody to plasma factor VIll/von Willebrand factor ( FVlll/vWF); however, no FVlll procoagulant activity has been associated with the protein produced by endothelial cells. Of its three biologic properties, only the FVlll procoagulant activity of purified plas...

GLucocorticoids inhibit the generation of leukocyte procoagulant (tissue factor) activity.

Intra-alveolar tissue factor pathway inhibitor is not sufficient to block tissue factor procoagulant activity.

The alveolar compartment in acute lung injury contains high levels of tissue factor (TF) procoagulant activity favoring fibrin deposition. We previously reported that the alveolar epithelium can release TF procoagulant activity in response to a proinflammatory stimulus. To test the hypothesis that the alveolar epithelium further modulates intra-alveolar fibrin deposition through secretion of an...

Activated factor XI increases the procoagulant activity of the extrinsic pathway by inactivating tissue factor pathway inhibitor.

Activation of coagulation factor XI (FXI) may play a role in hemostasis. The primary substrate of activated FXI (FXIa) is FIX, leading to FX activation (FXa) and thrombin generation. However, recent studies suggest the hemostatic role of FXI may not be restricted to the activation of FIX. We explored whether FXI could interact with and inhibit the activity of tissue factor pathway inhibitor (TF...